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수종의 암세포주와 섬유모세포주에서 taxol과 전리방사선이 세포독성과 prostaglandin생성에 미치는 영향
Elects of taxol and ionizing radiation on cytotoxicity and prostaglandin production in KB, RPMI-2650, SW-13 and L929

치과방사선 1998년 28권 1호 p.127 ~ 143

이건일, 유동수,

소속 상세정보

이건일 ( ) - 원광대학교 의과대학 통증치료실
유동수 ( ) - 서울대학교 치과대학 구강악안면방사선학

KMID : 0378219980280010127

Abstract

한국세포주은행에서 분양받은 3종의 인체 암세포주(KB. RPMI-2650, SW-13)와 1종의 생
쥐 섬유모세포주(L929)를 배양하여 다양한 농도(5, 50, 100, 200, 300, 500 nM)의 taxol로 처
리한 후 1일후와 4 일후에 MTT분석법으로 세포생존율을 구하여 세포독성 변화를 관찰하였
으며 또한 500 nM taxol처리군, 10 Gy 방사선조사군, 두 가지의 병행처리군에서 각세포주
의 세포생존율과 prostaglandin 중 PGE₂와 PGI₂의 양을 측정하
여 다음과 같은 결론을 얻었다.
1. KB 세포에서는 taxol처리 1일군에 비해 4일군에서 세포독성이 증가하였으며 양군 모두
에서 taxol 농도와 세포독성간에 높은 상관관계를 보였다(1일군 R=0.82741, 4일군
R=0.84655).
2. RPMI-2650 세포에서는 고농도 taxol처리군에서만 1일군에 비해 4일군에서 세포독성이
증가하였으며 4일군에서 taxol농도와 세포독성간에 매우 높은 상관관계를 보였다
(R=0.93917).
3. SW-13 세포에서는 저농도 taxol처리군을 제외하고는 1일군에 비해 4일군에서 세포독
성이 증가하였으며 양군 모두에서 taxol농도와 세포독성간에 높은 상관관계를 보이지 않았
다 (1일군 R=0.46362, 4일군 R=0.65425).
4. L929 세포에서는 저농도 taxol처리군에서 만 1일군에 비해 4일군에서 세포독성이 증가
하였으며 taxol농도와 세포독성간에 상관관계는 매우 낮았으며 (1일군 R=0.34237. 4일군 R=
0.23381) L929 1일군을 제외하고는 10Gy 방사선조사군에 비해 500nM taxol처리군에서 세포
독성이 컸으며 L929 1일군에서만 taxol의 방사선 증감효과를 관찰할 수 있었다.
5. 모든 암세포주에서는 병행처리 4일군에서 여러 개의 분열된 핵과 많은 부유세포들이
관찰되었으나 L929 세포에서는 더 높은 세포 충밀도를 보였으며 분열된 핵이나 부유세포는
관찰할 수 없었다.
6. PGE₂는 KB 세포에서 가장 많이 검출되었으며 모든 암세포주는 실험군
이 대조군에 비해 증가 하였으나 L929 세포에서는 감소하였고 SW-13 세포에서만 병행처리
군이 다른 군에 비해 유의성 있게 증가하였다. PGI₂는 RPMI-2650 세포에서
가장 많이 검출되었으며 SW-13 세포를 제외하고는 모두 증가하였고 RPMI-2650 세포에서
만 병행처리군에서 다른 군에 비해 유의성 있게 증가하였다.
#초록#
The author evaluated the effects of taxol, a microtubular inhibitor, as a possible
radiation.
sensitizer and the production of prostaglandins on three human cancer cell lines(KB,
RPMI-2650 and SW-13) and one murine cell line(L929). Each cell line was divided into
four groups(control, taxol only, radiation only and combination of taxol and radiation).
The treatment consisted of a single irradiation of 10 Gy and graded doses(5, 50, 100,
200, 300, 500 nM) of taxol for a 24-h period. The cytotoxicity of taxol alone was
measured at 1 day after(1-day group) and 4 days after(4-day group) the treatment. The
survival ratio of cell was analyzed by MTT (3-(4,5-dimethylthiazol-2-yl) -2,5-dimethyl
tetrazolium bromide) test. Prostaglandins(PGE2 and PGI2) were measured in the culture
medium by a radioimmunoassay.
The results obtained were as follows.
1. There was a significantly increased cytotoxicity of KB cells in 4-day group than
those in 1-day group. There was a high correlation between doses of taxol and cell
viability in both groups(1-day group R=0.82741, 4-day group R=0.84655).
2. There was a significantly increased cytotoxicity of RPMI-2650 cells treated with
high concentration of taxol in 4-day group than those in 1-day group, Also there was a
high correlation between doses of taxol and cell viability in 4-day group (R=0.93917).
3. There was a significantly increased cytotoxicity of SW-13 cells treated with high
concentration of taxol in 4-day group than those in 1-day group. However no high
correlation was observed between doses of taxol and cell viability in both groups(1-day
group R=0.46362, 4-day group R=0.65425).
4. There was a significantly increased cytotoxicity of L929 cells treated with low
concentration of taxol in 4-day group than those in 1-day group. At the same time,
there was a low correlation between doses of taxol and cell viability in both
groups(1-day group R=0.34237, 4-day group R=0.23381).
5. In 1-day group of L929 cells, higher cytotoxicities were observed in the groups
treated with 500 nM taxol than given 10 Gy radiation alone. L929 cells in 1-day group
alone showed a radiosensitizing effect by taxol.
6. In addition to L929 cells, all cancer cells treated with a combination of taxol and
radiation in 4-day group appeared to have some fragmented nuclei and to float on the
medium. In addition, L929 cells appeared to be more confluent.
7. The level of PGE₂ production was the highest in the control KB cells.
This appeared to increase in every experimental group of all three cancer cells except
L929 cells. There was a significantly increased production of PGE₂ in
SW-13 cells treated with a combination taxol and radiation compared to the other
experimental groups.
8. The level of PGI₂ production in the control group of RPMI-2650 cells
was the highest. This appeared to increase in every experimental group of all cells
except in SW-13 cells. This also increased significantly in RPMI-2650 cells treated with
a combination of taxol and radiation compared to the other experimental groups.

irradiation; MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-dimethyltetrazolium bromide); taxal(paclitaxel); prstalandins;